Complete Mitochondrial Genomes of Ancient Canids Suggest a European Origin of Domestic Dogs
University of California, Los Angeles
A lateral view of a Pleistocene wolf from the Trou des Nutons cave (Belgium), calibrated age of 26,000 years Before the Present. This wolf species was particularly large. Credit: Royal Belgian Institute of Natural Sciences
Wolves likely were domesticated by European hunter–gatherers more than 18,000 years ago and gradually evolved into dogs that became household pets, UCLA life scientists report.
"We found that instead of recent wolves being closest to domestic dogs, ancient European wolves were directly related to them," said Robert Wayne, a professor of ecology and evolutionary biology in UCLA's College of Letters and Science and senior author of the research. "This brings the genetic record into agreement with the archaeological record. Europe is where the oldest dogs are found."
The UCLA researchers' genetic analysis is published Nov. 15 in the journal Science and featured on the journal's cover.
In related research last May, Wayne and his colleagues reported at the Biology of Genomes meeting in New York the results of their comparison of the complete nuclear genomes of three recent wolf breeds (from the Middle East, East Asia and Europe), two ancient dog breeds and the boxer dog breed.
"We analyzed those six genomes with cutting-edge approaches and found that none of those wolf populations seemed to be closest to domestic dogs," Wayne said. "We thought one of them would be, because they represent wolves from the three possible centers of dog domestication, but none was. All the wolves formed their own group, and all the dogs formed another group."
The UCLA biologists also hypothesized at that conference that a now-extinct population of wolves was more directly related to dogs.
This photo provided by the Center for American Archaeology shows canine bones buried at the Koster site in Greene County, Ill. The fossil specimen at this site is dated to 8,500 years ago. A large DNA study suggests dogs arose from wolves in Europe some 19,000 to 32,000 years ago. Results were published online on Nov. 14, 2013 by the journal Science. / Del Baston, Center for American Archaeology
For the current study in Science, the researchers studied 10 ancient "wolf-like" animals and eight "dog-like" animals, mostly from Europe. These animals were all more than 1,000 years old, most were thousands of years old, and two were more than 30,000 years old.
The biologists studied the mitochondrial DNA of the animals, which is abundant in ancient remains. (Mitochondria are tiny sub-cellular structures with their own small genome.) By comparing this ancient mitochondrial DNA with the modern mitochondrial genomes of 77 domestic dogs, 49 wolves and four coyotes, the researchers determined that the domestic dogs were genetically grouped with ancient wolves or dogs from Europe—not with wolves found anywhere else in the world or even with modern European wolves. Dogs, they concluded, derived from ancient wolves that inhabited Europe and are now extinct.
Wayne said that that the domestication of predatory wolves likely occurred among ancient hunter–gatherer groups rather than as part of humans' development of sedentary, agricultural-based communities.
"The wolf is the first domesticated species and the only large carnivore humans ever domesticated," Wayne said. "This always seemed odd to me. Other wild species were domesticated in association with the development of agriculture and then needed to exist in close proximity to humans. This would be a difficult position for a large, aggressive predator. But if domestication occurred in association with hunter–gatherers, one can imagine wolves first taking advantage of the carcasses that humans left behind—a natural role for any large carnivore—and then over time moving more closely into the human niche through a co-evolutionary process."
The idea of wolves following hunter–gatherers also helps to explain the eventual genetic divergence that led to the appearance of dogs, he said. Wolves following the migratory patterns of these early human groups would have given up their territoriality and would have been less likely to reproduce with resident territorial wolves. Wayne noted that a group of modern wolves illustrates this process.
The Basenji represents one of the most divergent dog lineages in existence today. Genetic analyses of modern and ancient canids, including some of the oldest known dog remains, place the origin of modern dogs in Europe between 18,800 and 32,100 years ago. Credit: Science
"We have an analog of this process today, in the only migratory population of wolves known existing in the tundra and boreal forest of North America," he said. "This population follows the barren-ground caribou during their thousand-kilometer migration. When these wolves return from the tundra to the boreal forest during the winter, they do not reproduce with resident wolves there that never migrate. We feel this is a model for domestication and the reproductive divergence of the earliest dogs from wild wolves.
"We know also that there were distinct wolf populations existing ten of thousands of years ago," Wayne added. "One such wolf, which we call the megafaunal wolf, preyed on large game such as horses, bison and perhaps very young mammoths. Isotope data show that they ate these species, and the dog may have been derived from a wolf similar to these ancient wolves in the late Pleistocene of Europe."
In research published in the journal nature in 2010, Wayne and colleagues reported that dogs seem to share more genetic similarity with living Middle Eastern gray wolves than with any other wolf population, which suggested a Middle East origin for modern dogs. The new genetic data have convinced him otherwise.
"When we previously found some similarity between Middle Eastern wolves and domestic dogs, that similarity, we are now able to show, likely was the result of interbreeding between dog and wolves during dog history. It does not necessarily suggest an origin in the Middle East," Wayne said. "This alternative hypothesis, in retrospect, is one that we should have considered more closely. As hunter–gatherers moved around the globe, their dogs trailing behind probably interbred with wolves."
Wayne considers the new genetic data "persuasive" but said they need to be confirmed with an analysis of genetic sequences from the nucleus of the cell (roughly 2 billion base pairs)—a significantly larger sample than that found in mitochondrial DNA (approximately 20,000 base pairs). This is challenging because the nuclear DNA of ancient remains tends to become degraded.
While Wayne plans to pursue this follow-up research, he said he does not expect a nuclear genome analysis to change the central finding. However, he said, it will fill in more of the details.
"This is not the end-story in the debate about dog domestication, but I think it is a powerful argument opposing other hypotheses of origin," he said.
There is a scientific debate over when dogs were domesticated and whether it was linked with the development of agriculture fewer than 10,000 years ago, or whether it occurred much earlier. In the new Science research, Wayne and his colleagues estimate that dogs were domesticated between 18,000 and 32,000 years ago.
More information: "Complete Mitochondrial Genomes of Ancient Canids Suggest a European Origin of Domestic Dogs," by O. Thalmann et al. Science, 2013.
The geographic and temporal origins of the domestic dog remain controversial, as genetic data suggest a domestication process in East Asia beginning 15,000 years ago, whereas the oldest doglike fossils are found in Europe and Siberia and date to >30,000 years ago. We analyzed the mitochondrial genomes of 18 prehistoric canids from Eurasia and the New World, along with a comprehensive panel of modern dogs and wolves. The mitochondrial genomes of all modern dogs are phylogenetically most closely related to either ancient or modern canids of Europe. Molecular dating suggests an onset of domestication there 18,800 to 32,100 years ago. These findings imply that domestic dogs are the culmination of a process that initiated with European hunter-gatherers and the canids with whom they interacted.